Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 17)

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Table 3.2. Alternative running conditions for SDS-PAGE in the Mini-PROTEAN Tetra cell.
100 V 200 V
Run time 85–95 min 30–40 min
Expected current (per gel)
Initial 15–20 mA 25–50 mA
Final 5–10 mA 20–31 mA
Expected temperature 25°C 25–35°C
Outer buffer volume
1–2 Gels 2-gel mark 2-gel mark
3–4 Gels 4-gel mark 4-gel mark
Table 3.3. PowerPac power supply recommendations.
# Gels 100 V 200 V 300 V
1–2 Basic/HC/HV/Universal Basic/HC/HV/Universal Basic/HV/Universal
3–4 Basic/HC/HV/Universal Basic/HC/HV/Universal HV/Universal
4–8 HC/HV/Universal HC/HV/Universal Universal
9–10 HC/Universal HC/Universal Universal
11–12 HC/Universal HC/Universal Universal
Instruction Manual and Application Guide