Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 12)

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running buffer. Straighten the sides of the
wells, if necessary.
2. Set the electrode assembly to the open
position on a clean, flat surface (A).
3. Place the gel cassettes into the electrode
assembly. Two cassettes are required to create
a functioning assembly; when using 1 or 3
gels, use the buffer dam (included with the cell)
to complete the assembly.
a. Place the first cassette with the short plate
facing inward and so the gel rests at a 30°
angle away from the center of the electrode
assembly. Make sure the electrode assembly
remains balanced and does not tip over.
b. Place the second gel or buffer dam on the
other side of the electrode assembly, again
by resting the gel on the supports. The gels
rest at 30° angles, one on either side of the
electrode assembly, tilting away from the
center of the frame (B).
4. Gently push both gels toward each other,
making sure that they rest firmly and squarely
against the green gasket that is built into the
electrode assembly. Align the short plates to
ensure the edge sits just below the notch at
the top of the green gasket (C).
5. While gently squeezing the gel cassettes
(or cassette and buffer dam) against the green
gaskets (maintaining constant pressure and
with both gels in place), slide the green arms
of the clamping frame one at a time over the
gels, locking them into place (D,E).
6. The wing clamps of the electrode assembly lift
each gel cassette up against the notch in the
green gasket, forming a seal. Check again that
A
B
C
D E
Clamping frame
Short plate
Gasket
Notch
Gel cassette
Long plate
Gel support
Mini-PROTEAN Precast Gels