Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 33)

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Membrane
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Filter paper
Filter paper
Assembly of the Trans-Blot SD semi-dry cell .
Table 11 .1 . Placement of cassettes in the Trans-Blot Turbo cell .
Acceptable Unacceptable
Option 1 Option 2 Option 1 Option 2
Upper bay (A) 1 mini gel 2 mini gels -or- 1 midi gel 1 mini gel 2 mini gels -or- 1 midi gel
-and/or- -and/or- -and- -and-
Lower bay (B) 1 mini gel 2 mini gels -or- 1 midi gel 2 mini gels -or- 1 midi gel 1 mini gel
Table 11 .2 . Trans-Blot Turbo transfer protocols .
Protocol Name MW, kD Time, Min 1 Mini Gel 2 Mini Gels or 1 Midi Gel
STANDARD SD Any 30 Up to 1.0 A, 25 V constant Up to 1.0 A, 25 V constant
1.5 MM GEL Any 10 2.5 A constant, up to 25 V 1.3 A constant, up to 25 V
HIGH MW >150 10 2.5 A constant, up to 25 V 1.3 A constant, up to 25 V
LOW MW <30 5 2.5 A constant, up to 25 V 1.3 A constant, up to 25 V
MIXED MW 5–150 7 2.5 A constant, up to 25 V 1.3 A constant, up to 25 V
1 Mini TGX 5–150 3 2.5 A constant, up to 25 V N/A
11.2.4 Semi-Dry Transfer Using the Trans-Blot ® SD Cell
1. Equilibrate the gels in transfer buffer for 10–20 min.
2. Assemble the blot for transfer using the Trans-Blot SD semi-dry transfer system.
3. Connect the Trans-Blot SD cell to a PowerPac ™ Basic power supply and begin transfer at 25 V.
Optimum transfer efficiency is generally obtained in 30 min; smaller proteins (<30 kD) may transfer more
quickly, while proteins >150 kD may show increased transfer efficiencies at up to 60 min. Run times
longer than 60 min are NOT recommended for semi-dry transfers. Refer to the Trans-Blot SD Instruction
Manual (bulletin 1703940) or the Protein Blotting Guide (bulletin 2895) for additional information.
Instruction Manual and Application Guide