Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 42)

36 Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com
Mini-PROTEAN Precast Gels
Running buffer (1x) Dilute 100 ml 10x stock (catalog #161-0733) with 900 ml diH
2
O.
Sample buffer (5x) 89 mM Tris, 89 mM boric acid, 2 mM EDTA, pH 8.0, 12% Ficoll,
(catalog #161-0768) 0.01% bromophenol blue, 0.02% xylene cyanole FF, 7 M urea
Prepare Buffers
Prepare and Load Samples
Prepare Gels and
Assemble Electrophoresis Cell
Perform Electrophoresis
Remove the comb and tape from the gels and assemble the electrophoresis cell.
Fill the inner and outer buffer chambers with running buffer.
Denaturing Nucleic Acid PAGE (Mini-PROTEAN TBE-Urea Gels)
Component Amount
Sample 8 μl
Sample buffer
(catalog #161-0768) 2 μl
Total volume 10 μl
Load the appropriate amount of sample on the gel.
Connect the electrophoresis cell to the power supply and perform electrophoresis according to
the conditions in the table.
Table A.5. Running conditions for denaturing nucleic acid PAGE with one (1) Mini-PROTEAN TBE-urea gel in the
Mini-PROTEAN Tetra cell. Run conditions and times are approximate and assume a constant voltage of 200 V. When running
more than one gel, current will differ.
10% Gels 15% Gels
Power conditions 200 V constant 200 V constant
Expected current (per gel)
Initial 15 mA 15 mA
Final 10 mA 10 mA
Run time 45–60 min 60–75 min