Chapter 6: peptide analysis, 1 introduction, 2 mini-protean tris-tricine gels – Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis User Manual
Page 22: 1 gel composition, 2 gel selection guide, Peptide analysis
Peptide Analysis
6
6.1 Introduction
Mini-PROTEAN
®
Tris-Tricine peptide analysis gels are optimized for separating peptides and proteins
with molecular weight <10,000. Peptide-SDS complexes move more slowly through these gels, allowing
the faster SDS micelles, which normally interfere with peptide separations, to completely separate from
peptides. This enables resolution of distinct peptide bands.
6.2 Mini-PROTEAN Tris-Tricine Gels
6.2.1 Gel Composition
Gel buffer
1.0 M Tris-HCl, pH 8.45
Crosslinker
2.6% C
Stacking gel
4% T, 2.6% C
Storage buffer
1.0 M Tris-HCl, pH 8.45, NaN
3
Shelf life
12 weeks at 2–8°C; expiration date is printed on the packaging
6.2.2 Gel Selection Guide
Gel Percentage
Optimum Separation Range
16.5%
1.5–30 kD
10–20%
1–40 kD
Migration charts for protein standards on
Mini-PROTEAN Tris-Tricine gels.
16
Technical Support: 1-800-4BIORAD • 1-800-424-6723 • www.bio-rad.com