Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 39)

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Instruction Manual and Application Guide
Native PAGE (Mini-PROTEAN TGX Gels)
Running buffer (1x) Dilute 100 ml 10x stock (catalog #161-0734) with 900 ml diH
2
O.
Sample buffer Use native sample buffer (catalog #161-0738)
Prepare Buffers
Prepare and Load Samples
Prepare Gels and
Assemble Electrophoresis Cell
Perform Electrophoresis
Remove the comb and tape from the gels, rinse wells, and assemble the electrophoresis cell.
Fill the inner and outer buffer chambers with running buffer.
Component Volume
Sample 5 μl
Native sample buffer
(catalog #161-0738) 5 μl
Total volume 10 μl
Load the appropriate amount of sample on the gel.
Connect the electrophoresis cell to the power supply and perform electrophoresis according to
the conditions in the table.
Table A.2. Standard running conditions for native PAGE with one (1) Mini-PROTEAN TGX gel in the Mini-PROTEAN
Tetra cell . Run conditions and times are approximate and assume a constant voltage of 200 V. When running more than one
gel, current will differ but temperature and run time should be close to those listed.
Current (mA) at 200 V
Gel Initial Final Temperature Run Time
1 Gel (buffer to 2-gel mark)
7.5%
35–37 17–20 28–30°C 38–40 min
10%
12%
4–15% 50–55 25–28 30–33°C 30–34 min
4–20%
Any kD
™