Bio-Rad Mini-PROTEAN® TGX™ Precast Gels for 2-D Electrophoresis - Manual (Page 29)

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Detection
10
10.1 SDS-PAGE and Native PAGE Detection
Following electrophoresis, stain the gel with a total protein stain to visualize the proteins (Table 10.1).
Table 10 .1 . Total protein gel stains for use with Mini-PROTEAN gels .
Stain Sensitivity
(Lower
Limit)
Optimum
Protein Load
( µg/Band)
Advantages
Disadvantages
Imaging
Manual
Mini-PROTEAN TGX Gels
Coomassie
R-250 36–47 ng ~0.5 Laboratory
standard Requires
methanol
destaining Photography
with white light
or transmission
densitometry Consult
literature
Bio-Safe ™
Coomassie
8–28 ng ~0.5 Nonhazardous 4307051
Zinc stain 1 6–12 ng ~0.2 High contrast, fast,
reversible Negative SDS-
PAGE stain, must
be photographed 4006082
Silver
Stain Plus
™ kit
0.6–1.2 ng ~0.01 Sensitive, robust,
mass spectrometry
compatible Does not stain
glycoproteins
well LIT442
Silver stain 0.6–1.2 ng ~0.01 Stains complex
proteins (glyco- or
lipoproteins) Not mass
spectrometry
compatible LIT34
Dodeca ™
silver stain kit
0.5–1.2 ng ~0.1 Convenient
staining for a large
number of gels 4110150
Oriole ™
fluorescent
gel stain
1
~2 ng ~0.1 High sensitivity,
broad dynamic
range, simple
one-step protocol Fluorescence
visualization
with UV trans-
illumination 10017295
SYPRO
Ruby protein
gel stain 1–10 ng ~0.1 Broad dynamic
range Requires laser-
or LED-based
imaging
instrument for
maximum
sensitivity Fluorescence
visualization
with UV,
LED, or laser
scanning 4006173
Flamingo ™
fluorescent gel
stain
0.25–0.5 ng ~0.02 Broad dynamic
range, mass
spectrometry
compatible 10003321
Stain-free
imaging 2–28 ng ~0.5 Rapid (<5 min),
compatible with
blotting and mass
spectrometry,
simple protocol
with no additional
reagents Requires
tryptophan
residues in
proteins for
detection Fluorescence
using stain- free
compatible
imaging system 10014472
1 Do not use zinc stain or Oriole fluorescent gel stain to stain native gels.