ITC Avoximeter 1000E Whole Blood Oximeter - Manual (Page 8)

ITC AVOXimeter 1000E Operator’s Manual
2
The ITC AVOXimeter 1000E measures oxygenated hemoglobin [HbO
2
], deoxyhemoglobin [HHb],
methemoglobin [MetHb], and carboxyhemoglobin [COHb] directly, using novel optics and multiple
wavelengths. This reduces interference from dyshemoglobins and other interfering substances
such as fetal hemoglobin and bilirubin and minimizes the effects of hemolysis.
The measured values are used to calculate total hemoglobin [THb] and percent oxyhemoglobin
saturation [%Hb0
2
] of the sample, using the fractional method described below:
[THb] = [HbO
2
] + [HHb] + [MetHb] + [COHb]
[HbO
2
] x 100
[%HbO
2
] =
[HbO
2
] + [HHb] + [MetHb] + [COHb]
Oxygen content [O
2
] of the sample is then calculated:
1.39 x [THb] x [%HbO
2
]
[O
2
] =
100
where 1.39 is the ml of oxygen assumed to be carried by one gram of oxygenated hemoglobin
(Hϋfner’s Number). Depending on your facility protocols, the Hϋfner’s Number stored in the ITC
AVOXimeter
1000E can be set at any value in the range of 1.30 to 1.39 (see page 25).
Note: Below is a legend for oxygen saturation terminology used throughout this
document:
Abbreviation Term Units Displayed
THb Total Hemoglobin g/dl
HbO
2
Oxyhemoglobin g/dl
MetHb Methemoglobin g/dl
COHb Carboxyhemoglobin g/dl
HHb Reduced Hemoglobin g/dl
%HbO
2
Percent Oxyhemoglobin Saturation
(Fractional Oxygen Saturation) n/a
[O
2
] Oxygen Content ml/dl
Hemodynamic Calculations
Based on the results, hemodynamic calculations are performed as described below.
Oxygen Uptake
Cardiac Output
ml/min
=
[O
2
]
arterial
- [O
2
]
venous