ChemoMetec SCC-100 - Manual (Page 23)

2 Introducing the NucleoCounter
11
A fluorescent image of the content of the measurement chamber is thus recorded.
Example of such an image is shown in Figure 4. Each spot in the image represents a cell
containing DNA stained with PI.
Figure 4. Fluorescent image (from SomaticView PC software) of cell DNA
contained in the measurement chamber of an SCC-Cassette
2.1.2 Built-in image analysis
The NucleoCounter is equipped with advanced software for image analysis. This internal
software analyzes the recorded image and counts the number of nuclei in the image. As
the volume of the cassette measurement chamber and the dilution of the lyzate is
known, the cell density in the initial sample can be calculated. The concentration of
cells in the initial sample is presented in the NucleoCounter display. If the external
printer is connected to the instrument the result is printed, and if the optional software
(SomaticView) is used, the results and the recorded fluorescent image can be viewed.
The entire process takes approximately 30 seconds, from pressing “Run” to the
presentation of the result in the display of the NucleoCounter.
Somatic cell counting with the NucleoCounter are objective and the results are
independent of the operator.
2.2 The SCC-Cassette
The SCC-Cassette is a custom made disposable plastic device designed for optimal
sample handling and safe disposal. The cassette is shown in figure 5. The main features
of the SCC-Cassette are the piston, the flow system containing the fluorescent dye,
propidium iodide, and the measurement chamber.