ChemoMetec SCC-100 - Manual (Page 37)

5 Operation of the NucleoCounter
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and with the lid closed. The check determines the number of CCD-pixels that show
elevated signal levels. If the number of pixels is above a predefined limit then the
cassette insertion slit can be contaminated with an interfering particle, like a dust
particle. An error message is shown in the display and a cleaning of the insertion slit is
recommended (refer to chapter 6 Maintenance of NucleoCounter).
If a PC with SomaticView is connected to the instrument a contaminant is usually
observed as an object, which is stationary in all images.
To initiate a Zero Count Check type in “F50 + ↵”. Then the following text will appear in
the display:
0 count check
↵ Continue
Esc Abort
Figure 18 Zero Count Check, step 1
Press “↵” to continue. Now the instrument prompts you to remove any cassette from
the insertion slit:
Remove any
cassette
↵ Continue
Esc Abort
Figure 19 Zero Count Check, step 2
Press “↵” to continue. Now the instrument prompts you to close the lid in order to avoid
false light from entering the insertion slit during the Zero Count Check:
Close lid
↵ Continue
Esc Abort
Figure 20 Zero Count Check, step 3
Press “↵” to continue. Then a “Wait” message appears in the display:
wait...
Figure 21 Zero Count Check, step 3A