ChemoMetec SCC-100 - Manual (Page 25)

2 Introducing the NucleoCounter
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2.2.3 Sample volume
During production each cassette is marked with a dot code, which specifies the precise
depth or thickness of the measurement chamber of the cassette. The dot code is read
and decoded in the instrument during analysis and the volume analyzed is determined
by multiplying the imaged area with the sample depth of the cassette. The imaged area
is only dependent on the optics of the instrument. Therefore, the area is constant and
specific for each instrument.
2.2.4 Cassette handling
The built-in image analysis method of the NucleoCounter offers considerable stability,
when eliminating non-cellular objects from the image, including scratches and smears
on the cassette windows, and thus producing valid results even under extreme
conditions. On the other hand, the quality of the cell count is best assured by keeping
the windows of the cassette as clean as possible.
Caution! In order to avoid contaminating the measurement window it is important not
to touch the window when handling the cassettes. Be careful when attempting to wipe
off the surface of the cassette, in an attempt to remove any foreign object, since the
plastic material of the cassette can be scratched.
2.3 Lysis buffer
The lysis buffer for the lysing of somatic cells, Reagent C, is suitable for the sample
preparation of milk samples. It is highly effective and generally achieves virtually
instantaneous lysing of somatic cells in a milk sample. Nevertheless certain milk
samples, or preservation of milk samples, might require different preparation. Thus if
you encounter problems relating to insufficient lysing of somatic cells, please contact
ChemoMetec A/S for assistance.