Luminex xPONENT 3.1 - Manual (Page 108)

Luminex xPONENT 3.1 Software Manual
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2. Click Import Logo and then Open after navigating to the desired file to import a
graphic for the report header.
3. Click Clear Logo to remove the logo.
4. Click Save.
Adjusting the Sample Probe
Adjust the sample probe to ensure that it drops far enough into the well to acquire sample.
NOTE: Ensure that there is no liquid in the wells or reservoirs.
NOTE: You should adjust the probe height using row A unless you are
using the Automated Maintenance Plate. In that case, the probe
automatically moves to the specified well.
1. On the Home page, click Probe & Heater. The Probe & Heater tab opens.
2. Review the adjustment location for the well that you will use for alignment. Click a well
on the plate image if you want to use a different well. A blue pin notes the well selected
for alignment.
3. Remove the clear plastic shield that covers the sample probe area.
4. In a 96-well microtiter plate where overall height is no more than 0.75 inches (19mm),
place the appropriate alignment tool in the plate:
• For a standard plate with flat bottom wells - stack two of the larger (5.08 mm diameter)
alignment discs into the selected well.
• For a filter bottom plate - stack three of the larger (5.08 mm diameter) alignment discs
into the selected well.
• For a half-volume plate with flat-bottom wells - stack two of the smaller (3.35 mm
diameter) alignment discs into the selected well.
• For a round-bottom (U-bottom) plate - stack two of the smaller (3.35 mm diameter)
alignment discs into the selected well.
• For a plate with conical wells - place one alignment sphere into the selected well.
5. Verify that the microtiter plate is not warped. Warped plates can lead to incorrect probe
height adjustment.
6. Click Eject to eject the plate holder. Place the plate on the plate holder with well A1 in
the top left corner. If you are using the Automated Maintenance Plate, the probe
automatically moves to the specified location.
7. Click Retract to retract the plate holder.
8. Loosen the probe adjustment screw on the probe holder one-third to one-half turn. Pull
the sample probe upward until it touches the top of the adjustment slide. Tighten the
probe adjustment screw..
9. Click Move Probe Down to lower the sample probe.
10.Gently push the probe down until it just touches the top of the alignment discs or
sphere.
11.Tighten the probe adjustment screw.
NOTE: Use caution not to accidently move the probe up when tightening
the probe adjustment screw.
12.Replace the plastic shield that covers the sample probe area.
13.Click Eject to eject the plate holder. Remove the alignment tools from the plate.