Luminex xPONENT 3.1 User Manual

Page 9

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Table of Contents

Introduction .......................................................................................................................... 1

Software Packages ......................................................................................................... 1
About This Guide ............................................................................................................ 2
General Guidelines ......................................................................................................... 2

Biological Samples .................................................................................................... 2
Bead Handling ........................................................................................................... 2

Limitations ............................................................................................................ 3
Safety Precautions ............................................................................................... 3

Bead Concentration................................................................................................... 3
Repetitive xMAP Bead Measurements...................................................................... 3
Classification and Reporter Fluorochromes .............................................................. 4
Sample Volume ......................................................................................................... 4
Sample Dilution ......................................................................................................... 5
Reagents ................................................................................................................... 5
Gating ........................................................................................................................ 5
Plates......................................................................................................................... 5

Touring the Software............................................................................................................ 7

Log In .............................................................................................................................. 7
Help................................................................................................................................. 8
Log Off ............................................................................................................................ 8
Exit .................................................................................................................................. 8
Screen Elements............................................................................................................. 9
System Monitor ............................................................................................................. 10
Home ............................................................................................................................ 12
Samples ........................................................................................................................ 13

Samples Tab ........................................................................................................... 14
Create Sample Tab ................................................................................................. 15

Batches ......................................................................................................................... 16

Batches Tab ............................................................................................................ 16
Create a New Batch from an existing Protocol ........................................................ 18

Protocol Tab....................................................................................................... 18
Stds & Ctrls Tab ................................................................................................. 19
Plate Layout Tab ................................................................................................ 21

Create a New Batch From a New Protocol.............................................................. 24

Settings Tab ....................................................................................................... 24
Analytes Tab ...................................................................................................... 27
Stds & Ctrls Tab ................................................................................................. 31
Plate Layout Tab ................................................................................................ 32

Create a New MultiBatch......................................................................................... 33

Results .......................................................................................................................... 34

Current Batch Tab ................................................................................................... 34

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