Create a new batch from a new protocol, Settings tab – Luminex xPONENT 3.1 User Manual

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Luminex xPONENT 3.1 Software Manual

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Run Batch. Runs the batch and opens the Current Batch tab, where you can monitor the
batch as it runs.

Create a New Batch From a New Protocol

Click Create a New Batch from a new Protocol to create a new batch without using a
protocol. The Settings tab opens. Raw data results write to a .CSV file. You can define
parameters for samples, gates, regions, events, and commands.

NOTE:

If the batch spans more than one plate, the tray ejects
automatically when all defined wells have been acquired. A dialog
box displays prompting you to insert the next plate.

Settings Tab

Use this tab to name your new batch and configure acquisition settings.

FIGURE 12.

Settings Tab

This tab contains the following:

Name and Description boxes. Type a name and a description in the appropriate boxes.

Acquisition Settings. Use this section to set the following:

Bead Type - Select either MicroPlex (standard) or MagPlex (magnetic) beads.

Volume - This is the volume the instrument will aspirate into the system for analysis.
Type the desired sample volume in microliters. Use values from 10 to 200 µl for 96-well
plates. To avoid air intake, add at least 25 µl to the sample well in addition to the
sample size. The default value is 50 µl.

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