Luminex xPONENT 3.1 - Manual (Page 112)

Luminex xPONENT 3.1 Software Manual
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Setting Up Batches
A batch consists of a group of samples processed under control of a protocol. Batches
consist of protocols and samples for acquisition and can span more than one plate.
Protocols contain predefined commands that must be included in every batch acquisition.
You can also set up a custom batch that does not use a protocol.
You can group batches together as a multi-batch. Multi-batches can consist of any number
of batches that have been set up from different protocols and are processed
consecutively. Multi-batches can only be run on one plate.
Assay kit manufacturers may provide protocols in their kits, which are distributed on a CD.
Protocols typically include assay standards, controls, and maintenance commands (such
as washes or primes to acquire along with samples). Assay reagents are included in
assay kits. You must provide information about these reagents, such as lot numbers and
concentration values for the standards and assay controls.
NOTE: It is recommended that the manufacturer assay kit controls are
analyzed with each batch.
WARNING: Human and animal samples may contain biohazardous infectious
agents. Where exposure (including aerosol) to potentially
biohazardous material exists, follow appropriate biosafety
procedures and use personal protective equipment, such as
gloves, gowns, laboratory coats, face shields, or mask and eye
protection, and ventilation devices. Observe all local, state, and
federal biohazard handling regulations when disposing of
biohazardous waste material.
When setting up a batch, if the number of samples exceeds the wells in one microtiter
plate, you can add additional plates in the Add and Change Plate secondary window.
Additional plates are identified on the bottom of the plate image as Plate a of b, where a is
the plate number and b is the total number of plates.
Create a New Batch from an Existing Protocol
Read the instructions provided with the assay kit you are using.
1. Open the Batches page.
2. Click Create a New Batch from an existing Protocol.
3. Type the batch name in the Batch Name box.
4. Type a description about the batch in the Enter Optional Description box.
5. Click a protocol you wish to use in the Select a Protocol list. If the protocol uses
standards and/or controls, view the active reagents.
6. Click Next. If the protocol uses standards and/or controls, the next tab that appears is
the Stds & Ctrls tab. Otherwise, the Plate Layout tab appears. View the details of the
active reagents or apply different assay standards/controls or manually enter new
information. Select Next.
7. On the Plate Layout tab, assign well commands for this batch. See “Plate Layout Tab”
on page 21 for a complete description of the commands and options on this tab.
8. Click Run Batch to begin batch acquisition, or click Save to save batch information to
the Pending Batch list to be run at a later time.