Luminex xPONENT 3.1 - Manual (Page 48)

Luminex xPONENT 3.1 Software Manual
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Plate. A list of the available plates (if more than one).
WARNING: If using multiple plates, be careful to ensure that plates are used
in the proper order. Failure to do so can result in inaccurate data
and test results.
Histogram/Dot Plot. Displays real-time monitoring of batch sampling during acquisition.
Both the histogram and the dot plot have buttons that enable you to change the default
view:
• Click the maximize button to maximize the graph to fill the whole window. In the
maximized view, use the sliders to zoom in or zoom out. To return to the standard view,
click the minimize button.
• If the histogram or dot plot display have been changed to another view (e.g. Progress,
Well Report), click Default to return to the default histogram and dot plot displays.
• Dot Plot - The default location of the dot plot is the lower-right section of the Current
Batch tab. The dot plot is a graphical display of real-time data collection. Luminex
recommends using the default settings to collect data.
Right-click inside the dot plot area to access the following options:
• X Axis - Changes the X axis to the following: Doublet Discriminator, Reporter 1,
Classification 1, and Classification 2.
• Y Axis - Changes the Y axis to the following: Doublet Discriminator, Reporter 1,
Classification 1, and Classification 2.
NOTE: You can change the X axis of the dot plot for troubleshooting
purposes. Use only the default settings in all other instances.
• View - You can choose to view all events, or only gated events.
• Display Mode - Select either Logarithmic or Linear display. Logarithmic display is
the default. Click maximize for a magnified view of the histogram.
• Plot Type - Select either Density Plot or Decaying Plot. Density Plot is the default. It
displays a constant accumulation of events. Increasing density is indicated by
contrasting colors. The Decaying Plot plots only the last 100 acquired events.
• The Density Plot allows the visual elimination of data values which are determined to
be insignificant to the display. Luminex recommends you collect your data in Density
Plot mode to observe all collected events. You can only view the Decaying Plot during
batch acquisition.
Histogram. The default location of the histogram is the lower left section of the Current
Batch tab. The Doublet Discriminator (DD) displays on the X axis. Events display on the
Y axis. Doublets appear when two beads stick together, creating undesired results. When
you select the gate, two vertical dashed red lines appear. These represent gate positions
determined by the protocol. Once the gate is set, everything outside of it is ignored. For
batches created using an existing protocol, the gate cannot be changed while running the
batch. For new batches created from new protocols, the gate can be changed while
running the batch. Any changes made to the gate while running new protocol batches
immediately affect the visual display of the dot plot and histogram. In addition, changes
made to the gate while running new protocol batches affect the acquisition for all wells
initiated after the change is made. When viewing saved batch data, the gate can be
changed to visually inspect the effect. Only the visual display of the dot plot and histogram
is affected for saved batches, not the acquired data.