Luminex xPONENT 3.1 - Manual (Page 38)

Luminex xPONENT 3.1 Software Manual
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^ f) and the widely used Logic 5P regression fomula is calculated
as y = a + ((b-a)/(1 + ((x/c) ^ d )) ^ f).
NOTE: Luminex recommends Fit of All Standards as the most accurate
calculation of the curve fit.
Based on a range of quantitative, numerical results, a threshold range can be applied
to a quantitative analysis, for example, high, low, saturated, and expected.
• Allele Call - Sets analysis for an allele call. Analytes must put in groups of 2, 3, or 4
analytes.
• Min MFI Enabled - Select this box to enable a minimum MFI for the Allele Call
analysis. Type a value in this box to set the minimum MFI for analysis.
• Number of Standards - Click to type the number of standards for the protocol.
• Number of Controls - Click to type the number of controls for the protocol.
• Fit of all Standards - The standard curve will be determined by using each individual
standard replicate when calculating the standard curve. For example if one runs
duplicates of a 7 point standard curve, the software will calculate the standard curve by
using 14 points.
• Mean of Replicates - The standard curve will be determined by averaging the
individual standard replicates when calculating the standard curve. For example if one
runs duplicates of a 7-point standard curve, the software will calculate the standard
curve by using 7 averaged points.
• Analyze Results While Acquiring Samples - The software allows for real-time
viewing of the results as the instrument analyzes samples.
• Use External Analysis Program - Select this check-box to use a third-party program
to analyze the data. The Analysis Program list becomes active when this is selected.
• Analysis Program - Use this list to select which program to use for data analysis.
Cancel. Click to return to the main Batches tab.
Next:. Click to advance to the Analytes tab.