Luminex xPONENT 3.1 - Manual (Page 114)

Luminex xPONENT 3.1 Software Manual
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• To add well commands, click the appropriate wells and mark them as unknown,
standard, control, background, or wash. You can also delete commands that you’ve
added and change the starting location on the plate. If you wish to run in replicate,
change the Replicate Count to the appropriate number and the Grouping to your
preferred grouping method.
• To add maintenance commands, choose the desired command from the list. Choose
the well that you want to apply it to, and then choose Before well command or After
well command. If you are working with more than one plate, choose the Plate list.
Here, you can add a plate, change the order of the plates, and scroll through all plates.
• As you add commands to your plate, they appear in the Command Sequence list.
Here you can give each of your wells an ID. You can also import an ID list and move
your commands up and down to change the order in which they will be acquired.
For more information, see “Plate Layout Tab” on page 21.
17.Click Single Step if you want to run only the first sample, then pause acquisition.
18.Click Run Batch to run the acquisition, or Save to save the batch for a later time.
NOTE: If the batch spans more than one plate, the tray ejects
automatically when all defined wells have been acquired. A dialog
box displays prompting you to insert the next plate.
Create a Multi-Batch
A multi-batch is a set of batches that you want to process consecutively. You add batches
to the multi-batch from pending batches in your database. You can include as many
batches as you need. The software does not limit you to a certain number of batches per
multi-batch. You must ensure that the wells for each batch do not overlap, and that no
batch spans more than one plate. Results for each batch are saved as individual batch
files.
1. Open the Batches page.
2. Click Create a New Multi-Batch. The New MultiBatch tab opens.
3. In the Pending Batches list, choose the batch you want to add, then click Add Batch.
4. Click Run to run the multi-batch.
Import Batch
You need to import batches to the system only once. You must type lot information for the
standard and control reagents as specified in the protocol. This lot information is used for
every batch setup using the protocol until it is changed.
1. Open the Batches page.
2. Click Import. The Import Batch dialog box opens.
3. Click Browse to open the Select File dialog box. Navigate to the batch file you want to
import, then click Open.
4. Click OK in the Import Batch dialog box. The batch displays in the Pending Batches
list.
Export Batch
1. Open the Batches page.
2. In the Pending Batches section, click the batch you want to export, then click Export.
The Export Batch dialog box opens.
3. Click Browse The Select File dialog box opens.