Luminex xPONENT 3.1 - Manual (Page 61)

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Touring the Software
tabs to return to the main Protocols tab, or Back to return to a preceding tab. The View
option does not allow any changing of the protocol settings.
Settings Tab
Use this tab to name your new protocol and configure acquisition settings.
FIGURE 30. Settings Tab
This tab contains the following:
Name and Description boxes. Type a name and a description in the appropriate boxes.
Acquisition Settings. Use this section to set the following:
• Bead Type - Select either MicroPlex (standard) or MagPlex (magnetic) beads.
• Volume - This is the volume the instrument will aspirate into the system for analysis.
Type the desired sample volume in microliters. Use values from 10 to 200 µl for 96-well
plates. To avoid air intake, add at least 25 µl to the sample well in addition to the
sample size. The default value is 50 µl.
• Timeout - Select Enabled to enable a timeout. Acquisition stops once the timeout is
reached. Type a value from 1 to 250 seconds.
• XY heater - Select Enabled to enable the XY heater. In the box, type the desired value
in degrees Celsius. The temperature range is 35 to 60°C in 0.5 increments.
WARNING: Acquiring data before the heater has reached the proper
temperature can compromise test results.
• DD gating - Use this to set the minimum and maximum DD gating values. Use values
within the range of 0 to 32767. In the first box, type the minimum value. In the second