Metrohm 820 IC Separation Center - Manual (Page 41)

2.8 Precolumns
819 IC Detector / 820 IC Separation Center
33
2.8 Precolumns
The use of easily exchangeable precolumns protects the separating
columns and prolongs their lifetime. The precolumns available from
Metrohm are either real precolumns or precolumn cartridges, which are
used together with a cartridge holder. For the installation of a precol-
umn cartridge into the accompanying cartridge holder see the attached
leaflet.
The precolumn that is suitable for your separating column can be
found in the Metrohm IC Column Catalog which can be obtained
from your local Metrohm agency, the data sheet accompanying your
separating column, the product information about separating columns
that can be found under http://www.metrohm.com , ion chromatogra-
phy products, or let your agency advise you directly.
New IC precolumns are normally filled with solution and sealed at
both ends. Before the precolumn is installed in the system, it must be
ensured that this solution is freely miscible with the eluent used
(check manufacturer's specifications).
1 Connect precolumn
• Remove end caps from the precolumn.
• Fit compression fitting to the connection capillary 50
mounted to the injection valve (see Section 2.6).
• Screw column connection capillary 50 to precolumn.
• Cut off a small piece, ca. 5 cm, from the PEEK capillary
6.1831.010 and fit compression fittings to both ends (see
Section 2.6).
• Mount the prepared capillary to the outlet of the precolumn.
When you install the column, always ensure that this is inserted cor-
rectly in accordance with the flow direction (if existent) shown.
2 Rinse the precolumn
• Place a beaker beneath outlet capillary.
• Switch on 818 IC Pump in «IC Net» and rinse precolumn for
ca. 10 min with eluent.
• Switch off 818 IC Pump.