Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual (Page 10)

8
lmportant Considerations
Instruments and Software
The assays described in this manual are compatible with all currently
available Luminex-based life science research instruments. Assays
can be read and analyzed with either Bio-Plex Manager
™ software or
Luminex xPONENT software.
Assay Procedures
Pay close attention to vortexing, shaking, and incubation times and
to Bio-Plex
® reader PMT (RP1) setting, as these have been optimized
specifically for each assay panel.
Assay Quick Guide
Each assay kit includes a printed Bio-Plex Pro
™ Assay Quick Guide (bulletin
#10024986), which can be used to prepare and run a full 1 x 96-well assay
plate. Users can also download a copy at www.bio-rad.com/bio-plex.
Bead Regions
Bead regions for all analytes are listed in the Read Plate section.
Multiplexing Compatibility
Do not mix TGF-b assays with other Bio-Plex assay panels or reagent
kits. Protocols, reagents, and sample treatment conditions are not
compatible.