Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual (Page 16)

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Fig. 3. Preparing a fourfold dilution series with a single reconstituted standard.
RP1 (PMT) Setting for Standard Curves
The Bio-Plex ® 200 and 3D systems have two RP1 (PMT or photomultiplier
tube) setting options, while the Bio-Plex ® MAGPIX™ has no PMT
and therefore no PMT setting options. Instead, MAGPIX uses default
instrument settings similar to low PMT on the Bio-Plex 200 (Table 5).
The Bio-Plex Pro TGF-b assays were developed on the low PMT setting
using the Bio-Plex 200 system. Protocols using alternative standard
dilution series or PMT settings should be validated by the end user.
One
Reconstituted
Standard
72 150 150 150 150 150 150 150 150
S1 S2 S3 S4 S5 S6 S7 S8 Blank
Diluent, µl
128 50 50 50 50 50 50 50 Transfer Volume, µl
Instrument RP1 (PMT)
Bio-Plex 100, 200* Low, high
Bio-Plex 3D* Standard, enhanced
Bio-Plex MAGPIX* N/A, use default instrument settings
* Or similar Luminex-based system.
Table 5. Overview of PMT setting options on Bio-Plex systems.