Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual (Page 26)

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9. After the streptavidin-PE incubation step, slowly remove and discard
the sealing tape.
10. Wash the plate three times with 100 µl wash buffer.
11. To resuspend beads for plate reading, add 125 µl of assay buffer to
each well. Cover the plate with a new sheet of sealing tape. Shake at
room temperature at 850 ± 50 rpm for 30 sec, and slowly remove the
sealing tape. Ensure that the plate cover has been removed before
placing the plate on the reader.
12. Remove the sealing tape and read the plate using the settings
below. Refer to section 8, Read Plate, for details.
Note: Reading at alternative PMT settings on the Bio-Plex 100,
Bio-Plex 200, or Bio-Plex 3D requires validation by the end user to
ensure that results meet the user’s acceptance criteria.
Instrument RP1 (PMT) DD Gates Bead Events
Bio-Plex 100, 200* Low 5,000 (low), 25,000 (high) 50
Bio-Plex 3D* Standard Select MagPlex beads 50
Bio-Plex ® MAGPIX ™ * N/A, use default instrument settings Default
* Or similar Luminex-based system.
Table 13. Read plate using the appropriate instrument settings.