Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual (Page 21)

19
4. Dilute coupled beads to 1x by pipetting the required volume into the 15 ml
tube. Vortex.
Each well of the assay plate requires 2.5 μl (20x stock) adjusted to a
final volume of 50 μl in assay buffer.
5. Protect the beads from light with aluminum foil. Equilibrate to room
temperature prior to use.
Note: To minimize volume loss, use a 200–300 μl capacity pipet
to remove beads from the stock tube. If necessary, perform the
volume transfer in two steps. Do not use a 1,000 μl capacity pipet
and/or a wide bore pipet tip.
Preparing 1x coupled beads from 20x stock (includes 20% excess volume)
Table 7. Premixed panel or one singleplex assay.
Table 8. Mixing singleplex assays.
# of Wells 20x Beads, µl Assay Buffer, µl Total Volume, µl
96 288 5,472 5,760
48 144 2,736 2,880
20x Beads, µl 20x Beads, µl
# of Wells Singleplex #1 Singleplex #2 Assay Buffer, µl Total Volume, µl
96 288 288 5,184 5,760
48 144 144 2,592 2,880