Bio-Rad Bio-Plex Pro™ TGF-β Assays User Manual
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4. Dilute coupled beads to 1x by pipetting the required volume into the 15 ml
tube. Vortex.
Each well of the assay plate requires 2.5 μl (20x stock) adjusted to a
final volume of 50 μl in assay buffer.
5. Protect the beads from light with aluminum foil. Equilibrate to room
temperature prior to use.
Note: To minimize volume loss, use a 200–300 μl capacity pipet
to remove beads from the stock tube. If necessary, perform the
volume transfer in two steps. Do not use a 1,000 μl capacity pipet
and/or a wide bore pipet tip.
Preparing 1x coupled beads from 20x stock (includes 20% excess volume)
Table 7. Premixed panel or one singleplex assay.
Table 8. Mixing singleplex assays.
# of Wells
20x Beads, µl
Assay Buffer, µl
Total Volume, µl
96
288
5,472
5,760
48
144
2,736
2,880
20x Beads, µl
20x Beads, µl
# of Wells
Singleplex #1
Singleplex #2
Assay Buffer, µl
Total Volume, µl
96
288
288
5,184
5,760
48
144
144
2,592
2,880