Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual (Page 3)

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Introduction
Transforming Growth Factor (TGF-b) Assays
The TGF-b family of proteins plays an important role in a wide range of
cellular functions including proliferation, differentiation, migration, and
apoptosis. There are three known isoforms of TGF-b with overlapping
functions in normal physiology and in disease states such as cancer.
TGF-b1 promotes TH17 immune cell development and bone growth
and remodeling. TGF-b2 plays a vital role in embryonic development
and has been shown to suppress IL-2 dependent T-cell tumors. TGF-b3
regulates cell differentiation, adhesion, and extracellular matrix formation in
embryogenesis and wound healing.
Bio-Plex Pro ™ TGF-b assays are magnetic bead–based multiplex assays
designed to measure TGF-b1, TGF-b2, and TGF-b3 in human, mouse,
and rat sample matrices such as serum, plasma, urine, tissue culture
supernatant, and milk.
Multiplexing with Bio-Plex Pro Assays
Bio-Plex Pro assays enable researchers to quantify multiple protein
biomarkers in a single well of a 96-well plate in just 3 to 4 hours.
These robust immunoassays require as little as 12.5 µl serum or plasma,
or 50 µl cell culture supernatant or other biological fluid. The use of
magnetic (MagPlex) beads allows researchers to automate wash steps
on a Bio-Plex Pro (or similar) wash station. Magnetic separation offers
greater convenience, productivity, and reproducibility compared to
vacuum filtration.
For more information please visit www.bio-rad.com/bio-plex.