Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual (Page 28)

For reference, bead regions are shown in Table 14.
c. Click the Add button when the last analyte has been added and
click OK to save the new panel.
d. Highlight analytes from the Available list (left) and move to the
Selected list (right) using the Add button. To move all analytes at
once, simply click the Add All button.
e. If some of the analytes need to be removed from the Selected
list, highlight them and select Remove. If desired, it is possible to
rename the panel by clicking on Rename Panel and entering a
new panel name.
3. Click Format Plate and format the plate according to the plate layout
created in Section 1 (Plan Plate Layout). To modify the plate layout,
follow the steps below (see Figure 4).
a. Select the Plate Formatting tab.
b. Select the standards icon
S
and drag the cursor over all
the wells that contain standards. Repeat this process for
blanks
B
, controls
C
, and samples
X
.
4. Click Enter Standards Info in the Protocol Settings bar.
a. Enter the highest concentration of each analyte in the top row
(labeled S1) of the table. S1 concentration information is included
on the peel-off sticker provided with each vial of standards.
26
Analyte Bead Region
TGF-b1 13
TGF-b2 72
TGF-b3 66
Table 14. TGF-b assay bead regions.