Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual (Page 7)

5
Reagents Required but Not Supplied
TGF-b is secreted as part of a complex that causes it to be inactive.
It is necessary to expose samples to acidic conditions in order to activate
TGF-b. The following reagents are required:
n
1 N hydrochloric acid: To make 100 ml of 1 N HCl, add 8.33 ml of
12 N HCl slowly to 91.67 ml of deionized water, and mix well
n
1.2 N sodium hydroxide/0.5 M HEPES: To make 100 ml of 1.2
NaOH/0.5 M HEPES, add 12 ml of 10 N NaOH to 75 ml of deionized
water, and mix well. Add 11.9 g of HEPES (free acid, MW 238.3), mix
well, and bring the final volume to 100 ml with deionized water