Bio-Rad Bio-Plex Pro™ TGF-β Assays - Manual (Page 14)

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4. Prepare Standards
General Instructions
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It is essential to prepare standards exactly as described in this section.
Incorrect preparation may lead to low signal or variable measurements
from plate to plate
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The peel-off sticker provided with the standards lists the most
concentrated point on the standard curve (S1). Enter this information
into Bio-Plex Manager
™ software as instructed in section 8
Prepare a Diluent for Standards
1. Refer to Table 4 for recommended diluents based on different sample
types. As a general rule, reconstitute and dilute standards in a diluent
similar to the final sample type or sample matrix.
2. If samples are serum or plasma, mix 1 volume of Bio-Plex ®
standard diluent with 3 volumes of Bio-Plex sample diluent (each
supplied in the kit). The resulting solution is used for reconstitution
and subsequent dilution of standards. This results in a serum-matrix
based diluent that mimics the matrix in 1:16 diluted serum and
plasma samples.
Sample Type Diluent for Standard Add BSA
Serum and plasma Standard/sample diluent mix (1:3) None
Culture media, with serum Culture media None
Culture media, serum-free Culture media To 0.5% final* (w/v)
Lavage, lysate, other fluids Sample diluent To 0.5% final* (w/v)
* At least 0.5% final w/v BSA is recommended to stabilize analytes and reduce adsorption
to labware.
Table 4. Summary of recommended diluents for standards.