Bio-Rad Profinia Protein Purification System Kits - Manual (Page 10)

4.2 Instrument Startup, User Interface, and Method
Selection
Turn on the instrument using the recessed on/off switch at the right
lower panel. The Profinia home screen will appear. Make sure the cleaning
tray is in place and that filtered water is in buffer positions 1–7. Choose
Data/Utilities, Diag/Maint Functions, and Clean All Inlet and Outlet Lines.
This will flush ethanol or air from the system prior to starting a
chromatography run. After the system is rinsed press Home to return to
the Profinia home screen. Choose Bio-Rad Methods. The user interface
guides users through the setup and running of the preprogrammed
method. A brief description of each key screen and necessary actions to
take to perform a purification using the 51-D lysate are outlined below.
Select Method System Screen
The Select Method System screen
allows the user to choose among
different purification systems (IMAC,
GST, Desalting, Protein A&G, eXact).
(Figure 2)
•
IMAC
Select Method Type and Options
Screen
The Select Method Type and Options
screen allows the user to quickly
choose the method of purification,
number of samples to be purified,
and volumes for the affinity and/or
desalting cartridges. The selection in
Figure 3 are.
• Method type: Native IMAC +
Desalting
• Sample type: 1 sample
• Cartridge selection: 1 ml + 10 ml
6
Fig. 3. Method selection for native IMAC
and desalting application.
Fig. 2. Method selection for affinity
purification.
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