Bio-Rad Profinia Protein Purification System Kits - Manual (Page 19)

• Remove the buffer bottles in positions 1–7 and replace with bottles
that contain water (the bottles in the starter pack should be used for
this purpose)
• Remove the cartridges and connect the upper and lower cartridge
fittings. Press Start to begin the cleaning cycle
• If the long-term wash cycle is chosen, install 20% ETOH in buffer
positions 1–8, sample positions 1 and 2, and at the DI water inlet. The
ethanol will be used as a long-term storage solution in the lines
Section 8
Frequently Asked Questions and
Troubleshooting
For an extensive and comprehensive list of troubleshooting tips for
protein expression, sample prep, purification, and analysis, refer to the
Profinia Protein Purification Instrument Instruction Manual. Questions and
tips below focus on the use of the starter kits and solutions.
Proper Storage of Solutions and Kits
• Both kits can be stored at 22°C (room temperature) for short periods
of time, either upon receipt or during normal usage. Due to the sensi-
tivity of some of the more labile reagents (control lysate,
glutathione, GST cartridges), the kits should be kept at 4°C for
long-term storage
• Once opened and used with the instruments, all reagents should be
stored at 4°C for up to 3 months. After insertion into the instrument,
the solutions are no longer sterile and require 4°C storage
• After opening and use with the instrument, check the solutions for
particulates and clarity before reusing. If there is any indication of
particulates, contamination, or microbial growth, the solutions should
be discarded
Use of Excess Reagents
• The limiting reagent in the kit is the control lysate, which is sufficient
for two purifications yielding ~4 mg of target protein. The lysate can be
loaded at lower or higher amounts to mimic the purification of low-
level or high-level target proteins
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