Bio-Rad Profinia Protein Purification System Kits - Manual (Page 9)

Section 3
Equipment and Materials
3.1 Profinia Instrument and Accessories
Section 4
Setup and Running of the Profinia IMAC
Starter Kit
4.1 Reagent Preparation
Open the starter kit and remove the vial of lyophilized lysate. Dilute
6 ml of IMAC lysis buffer with 6 ml distilled water to make 12 ml of a 1x
solution. Resuspend the vial of lyophilized lysate with the 12 ml 1x lysis
buffer, swirl to mix, and let stand 5 min at room temperature to
completely dissolve. Note: Do not use lysis buffer from the GST starter kit
for IMAC purification because 10 mm EDTA is included in the GST lysis
buffer and will strip the nickel from the IMAC cartridge. Transfer 6 ml of the
resuspended sample to a 15 ml conical tube and place on ice until ready
to start purification. The remaining 6 ml of resuspended sample can be
frozen for later use and thawed once prior to purification. The frozen
sample should be used within 3 months.
The IMAC buffers and cartridges are ready for insertion into the
instrument and require no advanced preparation.
5
The instrument comes packaged
with nearly all materials necessary
for the setup and purification of the
control protein provided in the starter
kit. The instrument is shipped with all
the key bottles and plasticware
needed to run the system: waste
and diluent bottles, a bottle starter
pack, and a cleaning tray. See
Figure 1 for a picture of the
accessories. Materials not provided
in the package include standard 15
ml or 50 ml tubes that are required
for the sample and collection ports.
Most standard 15 and 50 ml tubes fit
the required dimensions of the
Profinia system.
Fig. 1. Accessories for the Profinia instrument.
Items shown include waste and diluent bottles (top),
bottle starter pack (left), sample cooling device (right),
cleaning tray (bottom), sample loop (bottom left), 15 ml
and 50 ml sample lids and buffer bottle lids (middle).
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