Bio-Rad Profinia Protein Purification System Kits - Manual (Page 16)

Section 6
Software and Analysis
6.1 Viewing Starter Kit Data
The standard software report presents key sample data collected throughout
the run. The sample name, fraction location and volume, and estimated
total protein eluted into collection tube D are displayed in the table in Figure 13.
The report can be formatted and printed in a variety of formats. The table
in Figure 13 highlights a partial section of the report.
Fig. 13. Standard software report.
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Software is an optional accessory to
the Profinia instrument and is
required to visualize chromatographic
data from the purification profile. A
representative chromatogram from
the 51-D control lysate purification is
shown in Figure 12.
The y-axis displays the absorbance
units (AU) and the x-axis displays
the run time (h:mm:ss). The first
peak traced by UV-1 illustrates the
unbound protein found in all frac-
tions: load (L), flowthrough (FT),
wash 1 and wash 2 (W-1, W-2), and
elution. The second peak traced by
UV-1 contains the affinity purified
protein, which is then diverted to the
desalting cartridge. The final peak
traced by UV-2 contains the purified
and desalted protein collected in the
elution fraction. A gel showing the
protein from each of the main
purification steps is shown in Figure 13.
Fig. 12. Representative chromatogram
displayed on Profinia software.
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