Bio-Rad Profinia Protein Purification System Kits - Manual (Page 5)

Section 1
Introduction
1.1 Background
The Profinia protein purification system includes a complete line of
consumables to use with an automated, user-friendly instrument for the
purification of immobilized metal affinity chromatography (IMAC) and
glutathione-S-transferase (GST) affinity-tagged proteins. The system has
been designed to use standard purification buffers, resins, and reagents
packaged into an easy-to-use kit. The two most common purification
applications, native IMAC and GST, have been packaged into starter kits
that contain all components necessary to purify a control affinity-tagged
protein. The buffers are supplied as concentrates and are diluted by the
instrument; no predilution is required. Profinia IMAC and GST starter kits
can be used after instrument installation and setup to familiarize the user
with the instrument or when troubleshooting purification problems. See
Section 10 for starter kit information and the complete list of Profinia products.
1.2 Product Information
Profinia starter kits are used to get started with the Profinia protein
purification system. The kits also help diagnose proper operation of the sys-
tem. The Profinia starter kits contain a lyophilized 51 kD dual-tagged protein
that contains an N-terminal GST sequence tag and a C-terminal tag with
histidine (His) residues.* Once resuspended in diluted equilibration buffer,
the lysate yields sufficient protein for two independent purifications. Each
kit contains a complete set of buffers and solutions (affinity, desalting,
cleaning, and storage solutions), reagents (glutathione), and cartridges (1 ml
affinity paired with a 10 ml desalting) required for two affinity purifications
only. However, additional Bio-Scale
™ Mini IMAC or GST cartridges can be
purchased separately, as the buffers provided in the starter kits are
sufficient to perform 10 purification runs. See Proper Storage of Solutions
and Kits on page 15. Table 1 summarizes the reagents contained within the
two starter kits available for the Profinia system.
* The C-terminal histidine tag contains alternating histidine units and is not recognized by
6x histidine antibodies.
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