Bio-Rad Profinia Protein Purification System Kits - Manual (Page 14)

Run Screens
A run is commenced by pressing the Start button. Once started, the
run in progress screen provides the approximate elution time for the
sample (typically 37 minutes for a demo run). Return to the instrument at
this time to retrieve the purified sample. (Figure 10)
Start run: This screen initiates the
purification run and notifies the user
how to view data in real time with
the Profinia data collection software
package.
Run in progress: A Run in Progress
screen is displayed for each method
selection. Features such as a status
bar, current step in the protocol
(for example, Equilibrate, Wash 1,
Elute), and expected time to sample
elution are all specified, so it is easy
to identify where the sample is at
any given point in the protocol.
Section 5
Setup and Running of the Profinia GST
Starter Kit
5.1 Reagent Preparation
Open the starter kit and remove the vial of lyophilized lysate. Dilute 6 ml
GST lysis buffer 1:1 with 6 ml distilled water to make 12 ml of a 1x solution.
Resuspend the vial of lyophilized lysate with the 12 ml of 1x lysis buffer,
swirl to mix, and let stand 5 min at room temperature to completely dissolve.
Transfer 6 ml of the resuspended sample to a 15 ml
10
Fig. 10. Start run and run in progress screens.
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