Bio-Rad Profinia Protein Purification System Kits - Manual (Page 8)

Table 3. Formulations for buffers and solutions provided in the GST
kits.
Profinia Supplied
Solution As Concentrated Formulation Working 1x Formulation Volume Position #
GST 2x 300 mM NaCl, 20 mM 150 mM NaCl, 10 mM Na
2
HPO
4
,
lysis buffer Na
2
HPO
4
, 10 mM EDTA, 5 mM EDTA, pH 7.4 125 ml N/A
pH 7.4
GST 2x 300 mM NaCl, 20 mM 150 mM NaCl, 10 mM Na
2
HPO
4
,
wash buffer Na
2
HPO
4
, 10 mM EDTA, 5 mM EDTA, pH 7.4 200 ml 1
pH 8.0
GST 2x 40 mM glutathione, 200 mM Tris, 20 mM glutathione, 100 mM Tris,
elution buffer 10 mM EDTA, pH 8.0 5 mM EDTA, pH 8.0 100 ml 3
Desalting 5x 685 mM NaCl, 13.5 mM KCI, 137 mM NaCl, 2.7 mM KCl,
buffer 21.5 mM Na
2
HPO
4
, 40.5 mM 4.3 mM Na
2
HPO
4
, 8.1 mM
KH
2
HPO
4
, pH 7.0 KH
2
PO
4
, pH 7.4 200 ml 4
(pH 7.4 upon dilution)
Cleaning 2x 1,000 mM NaCl, 500 mM NaCl, 50 mM Tris,
solution 1 100 mM Tris, pH 8.0 pH 8.0 125 ml 5
Cleaning 4x 2,000 mM NaCl, 500 mM NaCl,
solution 2 400 mM NaOAc, pH 4.5 100 mM NaOAc, pH 4.5 125 ml 6
Storage 2x 4% C
6
H
5
CH
2
OH 2% C
6
H
5
CH
2
OH
solution (benzyl alcohol) (benzyl alcohol) 200 ml 7
4
10006057E:4006073B.QXD 11/11/2009 10:40 AM Page 4