Bio-Rad Profinia Protein Purification System Kits - Manual (Page 6)

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Table 1. Profinia IMAC and GST starter kits.
Kit Catalog # Solutions Reagents Cartridges
Purification Desalting Storage
Buffers Buffer Solutions Lysate Glutathione Affinity Desalting
Native IMAC 620-0229
✔ ✔ ✔ ✔ ✔ ✔
starter kit
GST starter kit 620-0230
✔ ✔ ✔ ✔ ✔ ✔ ✔
1.3 Storage Conditions
Profinia starter kits are shipped at room temperature and can be safely
stored for short periods (up to 2 weeks) at room temperature. For long-term
storage, the kits should be stored at 4°C. The individual components and
outside kit box labels provide exact expiration dates.
Section 2
Native IMAC and GST Starter Kits
2.1 Native IMAC Component Descriptions
The affinity buffers provided in the native IMAC kit are formulated from
potassium salts and buffers, and contain increasing concentrations of
imidazole for optimized washing and eluting of His-tagged proteins. Native
IMAC buffers are used for proteins soluble in
E. coli lysates and that have
accessible tags containing histidine residues. Table 2 provides a list of
buffer compositions. The native lysis buffer is used for preparing
sample lysates; is not used in the instrument. This buffer can be used for
subsequent purifications of target protein outside of the starter kit
applications.
The IMAC cartridges are shipped in 20% ethanol and are packed with
1 ml Profinity ™ IMAC resin. The desalting cartridges are provided in
20 mM Bis-Tris, pH 6.5 with 0.05% azide as a preservative. The control
lysate is provided as a lyophilized sample prepared from 50 ml
E. coli
culture. When resuspended in 12 ml of 1x IMAC lysis buffer, ~4 mg of
tagged target protein can be purified from a 5 ml sample load. All
components supplied with the native IMAC starter kit are shown in Table 2.
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