Bio-Rad Profinia Protein Purification System Kits User Manual

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Table of Contents

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Section 1 Introduction............................................................1

1.1 Background ....................................................................1
1.2 Product

Information..........................................................1

1.3 Storage

Conditions ..........................................................2

Section 2 Native IMAC and GST Starter Kits ........................2

2.1

Native IMAC Component Descriptions ............................2

2.2

GST Component Descriptions..........................................3

Section 3 Equipment and Materials ......................................5

3.1

Profinia Instrument and Accessories ................................5

Section 4 Setup and Running of the Profinia

IMAC Starter Kit ....................................................5

4.1 Reagent

Preparation ........................................................5

4.2

Instrument Startup, User Interface, and
Method Selection ............................................................6

Section 5 Setup and Running of the Profinia

GST Starter Kit ....................................................10

5.1 Reagent

Preparation ......................................................10

5.2

Instrument Startup, User Interface, and
Method Selection ..........................................................11

Section 6 Software and Analysis ........................................12

6.1

Viewing Starter Kit Data ................................................12

6.2

Analysis of Purified Protein ............................................13

Section 7

Shutdown, Cleaning, Storage Sequence,
and Data Export ..................................................14

Section 8

Frequently Asked Questions and
Troubleshooting ..................................................15

Section 9

Legal Notices ......................................................16

Section 10 Ordering Information ..........................................17

10006057E:4006073B.QXD

11/11/2009

10:40 AM

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