Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 17)

7. Plot all 8 spectra and select the IgG peak using the peak selection tool.
Note: Spots with high concentrations (spots A, C, E, and G) alternate with those with low
concentrations (spots B, D, F, and H). Due to the different concentrations on different spots, it is
critical to export the peak data with the spots in alphabetical order from A to H.
8. Export the data following the instructions in Appendix C and selecting parameters for export
in the following order:
• Array bar code
• Spot name
• Substance mass
• Intensity
• Resolution
• S/N
9. Paste the data into the ProteinChip SELDI OQ form, data sheet “Test 1 Detector Sensitivity”.
10. The spreadsheet indicates if the test passed or failed.
11. If test 1 fails, run the detector calibration procedure (maintenance procedure), and repeat the
test. If the test fails a second time, contact technical support.
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