Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 7)

Chapter 2
Use of the ProteinChip OQ Kit
2.1 Overview
This chapter outlines the protocols used to perform the procedures and tests that comprise the
ProteinChip OQ kit. Please note the following:
• Export data acquired during testing to the ProteinChip SELDI OQ form using the export
instructions described in Appendix C
• Protocol transfer from the CD to ProteinChip data manager software needs only to occur
once; however, new files must be created each time a test is run
• Neither spreadsheets nor protocols are write-protected. Overwriting may result in erroneous
results and calculations
• Always perform the two maintenance procedures before running any of the tests
• Maintenance procedure 2 (detector calibration) is designed to standardize and stabilize
detector performance over time, by resetting any drift in the response due to natural component
aging. First or delayed use of this procedure may alter system response after calibration. Do
not run this procedure within a series of related experiments if it has been more than two
weeks since the last detector calibration
• All tests require peak measurements that are compared to specification. Incorrect peak
selection may lead to erroneous results. While selecting peaks for measurement, zoom in on
them so that they resemble the peaks in the examples provided
• When running single ProteinChip arrays on the ProteinChip instrument, Enterprise Edition,
always fill empty locations in the cassette with blank, expired, or used arrays. Blank arrays
are available as components of the ProteinChip cassette-compatible bioprocessor (catalog
#C50-30011)
• The ProteinChip detector calibration array and ProteinChip peptide standard array can be
used multiple times. After opening, store these arrays in a dry and dark place and in their
original packaging
• When running protocols, you specify how many sections (partitions) the spot is divided into
for sampling. You also specify which of these partitions should be used. For spectra intended
for analysis, no more than four ("of 4") partitions should be used, with 10 shots/pixel
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