Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 22)

2.4.3 Test 3 –– Resolution at 1 kD
This test uses the ProteinChip peptide standard array to measure the resolution of Arg-8-vasopressin.
To do this, a higher-resolution, lower source mode (15 kV) is employed rather than the default
high-sensitivity source mode (voltage 25 kV).These measurements are compared to a specification,
and a pass/fail disposition is obtained.
Caution: Spectra taken using one source mode should not be compared either qualitatively or
quantitatively to spectra taken using other source modes. Spectra are only comparable within
modes.
Array Type ProteinChip peptide standard array
Usage Information Each test uses 1 partition on all 8 spots on the array. The array
can be used up to 20 times (5 times on each of 4 partitions).
Store the array in the original packaging and record usage
information in the usage chart provided and on the packaging.
Laser optimization steps use 1 of 15 partitions, and these reads
do not need to be recorded or monitored.
Recommended Testing Weekly
Frequency
Expected Testing Time 30 min to 1 hr
Pass/Fail Specification Pass if resolution is greater than 1000
1. Insert the ProteinChip peptide standard array into the instrument. If you are using an
Enterprise Edition instrument, place the array into a cassette, fill the remaining slots in the
cassette with blank or used arrays, and place the cassette into the instrument.
2. In ProteinChip data manager software, click Start and open the Protocol Mode tab.
3. Select Instrument > Preferences. In the Preferences dialog, click “…”. The Select Folder
dialog opens.
4. Select Projects > OQ_####/ Date/Test 3 Resolution 1 kD, where #### is the serial number
of the instrument.
5. Optimize the laser intensity. Run protocol “Test 3 Resolution 1 kD” on any partition (“n”) of 15
on spot D. Adjust the laser energy until the height of the insulin peak at 5.96 kD is 100–200 µA.
This optimization step does not need to be repeated on subsequent runs of test 3 unless the
peak intensity falls too low.
6. Run the same protocol on partition “n” of 15 on spot D. Change the laser energy until the
height of the Arg-8-vasopressin peak at 1 kD is 100–200 µA.
7. Ensure that a peak intensity of 50–500 µA is visible on each spot. If peak intensities appear
out of this range, change the laser energy and run the test again.
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