Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 29)

9. Zoom in and check that all 7 peaks in all 8 spectra are marked correctly. Failure to do so
may result in incorrect results. Close the plot.
Mass Accuracy — External Calibration Test
Perform this test BEFORE the internal calibration test.
1. Click on the spectrum for spot D.
2. Select Spectra > Calibrate > Apply Calibration Protocol.
3. Select OQ Mass Cal.
4. Click OK. The spectrum flag changes from yellow to green.
5. In the main window, make sure that only the spectrum from spot D is selected. Click the
Copy Calibration button. The spectrum flag on spot D reverts to yellow.
6. Select all 8 spectra and click the Apply Calibration button. (Do not select the first
optimization spectrum.)
7. Export the data following the instructions in Appendix C and selecting the parameters for
export in the following order:
• Array bar code
• Spot name
• Substance mass
• Intensity
• Resolution
• S/N
• Peak #
8. Paste the data into the ProteinChip SELDI OQ form, data sheet “Test 4 Mass Accuracy”.
Paste the data into cell M41.
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