Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 25)

2.4.4 Test 4 — Mass Accuracy
This test uses the ProteinChip peptide standard array to test the mass accuracy of the system’s
internal and external calibrations. These measurements are compared to a specification, and a
pass/fail disposition is obtained.
Array Type ProteinChip peptide standard array
Usage Information Each test uses 1 partition on all 8 spots on the array. The array
can be used up to 20 times (5 times on each of 4 partitions).
Store the array in the original packaging and record usage
information in the usage chart provided and on the packaging.
Laser optimization steps use 1 of 15 partitions, and these reads
do not need to be recorded or monitored.
Recommended Testing Weekly
Frequency
Expected Testing Time 30 min to 1 hr
Pass/Fail Specification External calibration: Pass if average mass within 0.1% of
calibrant mass and pooled CV of <0.05
Internal calibration: Pass if average mass within 0.01% of
calibrant mass and pooled CV of <0.01
1. Insert the ProteinChip peptide standard array into the instrument. If you are using an
Enterprise Edition instrument, place the array into a cassette, fill the remaining slots in the
cassette with blank or used arrays, and place the cassette into the instrument.
2. In ProteinChip data manager software, click Start and open the Protocol Mode tab.
3. Select Instrument > Preferences. In the Preferences dialog, click “…”. The Select Folder
dialog opens.
4. Select Projects > OQ_####/ Date/Test 4 Mass Accuracy, where #### is the serial number
of the instrument.
5. Optimize the laser intensity. Run protocol “Test 4 Mass Accuracy” on any partition (“n”) of 15
on spot D. Adjust the laser energy until the height of the insulin peak at 5.96 kD is 100–200 µA.
This optimization step does not need to be repeated on subsequent runs of test 4 unless the
peak intensity falls too low.
6. Run protocol “Test 4 Mass Accuracy”. Run on partition 1 of 1 for all 8 spots on chip. Do not
change any values.
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