Bio-Rad ProteinChip Qualification and Calibration Kits - Manual (Page 18)

2.4.2 Test 2 — Mass Drift and Resolution 5.96 kD
This test uses the ProteinChip peptide standard array to measure the mass drift and resolution
of insulin. These measurements are compared to a specification, and a pass/fail disposition is
obtained.
Array Type ProteinChip peptide standard array
Usage Information Each test uses 1 partition on all 8 spots on the array. The array
can be used up to 20 times (5 times on each of 4 partitions).
Store the array in the original packaging and record usage
information in the usage chart provided on the packaging. Laser
optimization steps use 1 of 15 partitions, and these reads do
not need to be recorded or monitored.
Recommended Testing Weekly
Frequency
Expected Testing Time 20 min to 1 hr
Pass/Fail Specification Mass drift: pass if less than 7 Da
Resolution: pass if average is greater than 750
1. Insert the ProteinChip peptide standard array into the instrument. If you are using an
Enterprise Edition instrument, place the array into a cassette, fill the remaining slots in the
cassette with blank or used arrays, and place the cassette into the instrument.
2. In ProteinChip data manager software, click Start and open the Protocol Mode tab.
3. Select Instrument > Preferences. In the Preferences dialog, click “…”. The Select Folder
dialog opens.
4. Select Projects > OQ_####/ Date/Test 2 Mass Drift and Resolution 5.96 kD, where ####
is the serial number of the instrument.
5. Optimize the laser intensity. Run protocol “Test 2 Mass Drift and Resolution 5.96 kD” on any
partition (“n”) of 15 on spot D (for example, 1 of 15, 2 of 15, 3 of 15, etc). Adjust the laser
energy until the height of the insulin peak at 5,963 Da is 100–200 µA. This optimization step
does not need to be repeated on subsequent runs of test 2 unless the peak intensity falls
too low.
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