2 storage and handling – Bio-Rad ProteinChip Qualification and Calibration Kits User Manual

Page 6

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Table 1. Summary of procedures and tests (

continued).

R

Reecco

om

mm

meen

nd

deed

d

C

Co

on

nssu

um

maab

bllee

E

Essttiim

maatteed

d T

Tiim

mee

T

Teesstt

FFrreeq

qu

ueen

nccyy

U

Usseed

d

R

Reeq

qu

uiirreed

d

D

Deessccrriip

pttiio

on

n

T

Teesstt 2

2

Mass drift and

Weekly

ProteinChip

20 min to 1 hr

This test uses the ProteinChip

resolution at

peptide

peptide standard array to

5.96 kD

standard array

measure the mass drift and
resolution of insulin. These
measurements are compared to a
specification, and a pass/fail
disposition is obtained.

T

Teesstt 3

3

Resolution at

Weekly

ProteinChip

20 min to 1 hr

This test uses the ProteinChip

1 kD

peptide

peptide standard array to

standard array

measure the resolution of
Arg-vasopressin. These
measurements are compared to
a specification, and a pass/fail
disposition is obtained. The test
is run in higher-resolution
(lower source voltage) mode.

T

Teesstt 4

4

Mass

Weekly

ProteinChip

30 min to 1 hr

This test evaluates the mass

accuracy

peptide

accuracy when compared to

standard

internal and external

array

calibrations. These measurements
are compared to a specification,
and a pass/fail disposition is
obtained.

1.2 Storage and Handling

Store the arrays supplied in the kit in the original packaging at room temperature and in a dry
and dark location. Each array is individually wrapped. Do not open the array packaging until
ready to use. Take care when handling the ProteinChip arrays to avoid contaminating or disturbing
the matrix crystals on the surface of the arrays.

3

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