YSI 9600 - Manual (Page 17)

• Locate the 60 mL plastic syringe that was supplied with your Model 9600 in the 9615 Syringe Kit and
remove the plunger. Clamp the syringe to a ringstand with the Luer Lock bottom fitting “down” and
the syringe opening “up”.
• Remove the Luer Lock plug from the side port on the reagent bag and make sure that the pinch clamp
on the middle port of the bag is open. CAUTION: Make sure that you save the side port plug for
resealing the bag after filling.
• Attach the reagent bag via the side port to the Luer Lock fitting at the bottom of the syringe and tighten
securely. See the pictures below for the proper set-up to begin filling reagent bags.
• Make sure that the pinch clamp on the middle port of the bag is in the “open” position.
• Pour the appropriate reagent into the 60-mL syringe and allow it to drain into the reagent bag. Repeat
the addition of reagent until the bag is full and reagent overflows slightly out of the middle port of the
bag.
• Close the pinch clamp on the middle port of the reagent bag so that no solution can escape via this
route.
• Carefully remove the syringe from the side port of the reagent bag and seal the side port with the plug
which was set aside above. With the bag completely sealed, invert the bag back and forth several
times to loosen any bubbles that might be present.
• Now hold the bag in your hand with the connectors “up” and move it slightly back and forth until any
bubbles present are near the side Luer Lock fitting. Loosen the Luer Lock plug slightly (Do not
remove it) and squeeze the bag gently to expel any bubbles out through the port as shown in the picture
below. Then quickly retighten the Luer Lock plug. See the picture below.
YSI Environmental 17 Model 9600 Nitrate Monitor