YSI 9600 - Manual (Page 20)

• Rinse and fill the syringe assembly with 10 mL of purified water and repeat the injection procedure
through the column to rinse out the acid. Again, the injection process should take approximately 15
seconds.
• Rinse the syringe assembly with purified water and fill it with the 2 % (w/v) copper sulfate solution
prepared in 2.5.1 above. Connect the syringe assembly to the cadmium column and slowly inject 5 mL
of the reagent into the column while holding the open end of the column over a beaker. The injection
of the 5 mL aliquot of reagent should take approximately 30 seconds. Do not remove the syringe
assembly (which still should contain 5 mL of solution) from the cadmium column .
• Reinsert the threaded plug in the open end of the column assembly.
• Set the entire assembly aside for at least 15 minutes to allow proper activation. No damage to the
column will occur if the assembly is left for several hours in this state.
• After the initial activation, remove the threaded plug from the open end of the column, slowly inject
the remaining 5 mL of the copper sulfate solution into the cadmium column and again set the assembly
aside for 15 minutes or longer after reinserting the plug in the open end of the column.
• Remove the syringe from the assembly and rinse it with purified water. Remove the threaded plug
from the other end of the column assembly .
• Fill the syringe with 10 mL of the ammonium chloride buffer solution which was prepared as
described in 2.3.3 above. Inject all of the buffer reagent through the cadmium column at a rate so that
the entire injection takes about 15 seconds.
• Remove the syringe from the column assembly and reinsert the threaded plugs in each end of the
assembly.
The cadmium column is now activated and ready for installation.
2.6 INSTALLING THE CADMIUM COLUMN
The next step in setting up for deployment is to install the cadmium column in the carousel of the nitrate
analyzer. Remove the carousel from the outer shell of the analyzer and place it on the floor or a bench top
in the vertical position supported by its three legs.
YSI Environmental 20 Model 9600 Nitrate Monitor