Metrohm 881 Compact IC pro – Cation - Manual (Page 74)

5.7 Inline sample preparation
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881 Compact IC pro – Cation
5.7 Inline sample preparation
To protect the separation column (see Chapter 3.17, page 44) against for-
eign particles which can affect the separating efficiency, we recommend
that all samples undergo a microfiltration (filter 0.45 µm). The ultrafiltra-
tion cell can be used for filtration (see manual of the IC Equipment for
Ultrafiltration).
Matrix-loaded samples (e.g. blood, oil) should be prepared for the meas-
urement by means of dialysis (see manual on the IC Equipment for Dialy-
sis).
If the concentration of the sample is too high, the sample should be dilu-
ted before feeding (see documentation on the IC Equipment for Sample
Dilution).
For an overview of all Metrohm inline sample preparation methods go to
the following website:
http://misp.metrohm.com
5.8 Rinsing the sample path
Before a new sample can be measured, the sample path must be rinsed
with it so that the measuring result is not falsified by the previous sample
(Sample carry-over).
In the case of automated sample feeding, the rinsing time should be at
least 3 times the transfer time.
The transfer time is the time required by the sample to flow from the sam-
ple vessel to the end of the sample loop. ##NO_MATCH##.
Ascertaining the transfer time
To ascertain the transfer time, proceed as follows:
1 Emptying the sample path
Pump air through the sample path (pump tubing, tubing connec-
tions, sample loop) for several minutes until all liquid is displaced by
the air.
2 Aspirating the sample and measuring time
Aspirate a sample typical for the later application and use a stop
watch to measure the time required by the sample to travel from the
sample vessel to the end of the sample loop.