Ocean Optics OOIBase32 - Manual (Page 107)

A: Experiment Tutorials
7. Click the Save icon on the toolbar or select File | Save | Processed from the menu bar to save the
spectrum to disk.
Note
If you change any sampling variable (integration time, averaging, smoothing, fiber size,
etc.), you must store a new dark and reference spectrum.
Time Acquisition Experiments
OOIBase32 allows you to perform time acquisition experiments. Time acquisition experiments track
processes, perform kinetic analyses, and monitor spectral events all as a function of time. You can collect,
as a function of time, spectral data from up to six single wavelengths (known as Channels A through F)
and up to two mathematical combinations of these wavelengths (known as Combinations 1 and 2).
Additionally, you can acquire data in any mode (transmission, absorbance, etc.).
► Procedure
Follow the steps below to perform a time series experiment in OOIBase32:
1. Enter scope mode and store a reference spectra and dark spectra.
2. Choose the measurement mode (absorbance, transmission, etc.) and select Time Acquisition |
Configure | Configure Time Channels from the menu bar to access the Time Acquisition
Channel Configuration screen.
Proceed to the Configuring the Time Acquisition Channel Configuration Screen section below.
Configuring the Time Acquisition Channel Configuration Screen:
1. Perform the following steps on the Time Acquisition Channel Configuration screen:
a. Select Enabled to set the time acquisition calculation for the wavelength. The time
acquisition process will not calculate data if you do not select this option for at least
b. Select Plotted to see a real-time graph of the acquired data in a spectral window.
c. Select a Spectrometer Channel for the time acquisition process
d. Specify the analysis wavelength in the Wavelength (nm) box.
e. Specify the number of pixels around the analysis wavelength to average in the
Bandwidth (pixels) box.
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