Ocean Optics OOIBase32 - Manual (Page 98)

A: Experiment Tutorials
Once you install the hardware, configure the software, and establish your sampling system, you are ready
to take measurements.
This section details five types of experiments:
• Absorbance Experiments
• Transmission Experiments
• Reflection Experiments
• Relative Irradiance Experiments
• Time Acquisition Experiments
The type of measurement you will take determines the configuration of the sampling optics for your
system. Furthermore, your choice of reference and data analysis determines how the OOIBase32 presents
the results.
Note
For each measurement, you must first take a reference and dark spectrum. After you take
a reference and a dark spectrum, you can take as many measurement scans as needed.
However, if you change any sampling variable (integration time, averaging, smoothing,
fiber size, etc.), you must store a new dark and reference spectrum.
Application Tips
If the signal you collect is saturating the spectrometer (intensity greater than 4000 counts), you can
decrease the light level on scale in scope mode by:
• Decreasing the integration time
• Attenuating the light going into the spectrometer
• Using a smaller diameter fiber
• Using a neutral density filter with the correct optical density
If the signal you collect has too little light, you can increase the light level on scale in scope mode by:
• Increasing the integration time
• Using a larger diameter fiber
• Removing any optical filters
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