Ocean Optics OOIBase32 - Manual (Page 64)

7: Spectrometer Menu Functions
Software Configuration:
1. Open OOIBase32 and select Spectrometer | Configure.
2. Select the Reference Monitoring tab.
3. Locate the channel (in the Spectrometer Channel field) to use for your experiment.
4. Select the Reference Monitoring tab and select a Reference Channel.
5. Select Wavelength-by-Wavelength under Reference Type.
6. Store a dark and a reference spectrum of the sample in Scope mode.
Note
You must perform Step 6 after configuring reference monitoring.
7. Change to the appropriate spectral view mode in OOIBase32 (or stay in Scope mode).
OOIBase32 now will automatically correct for drift and reflect any correction in the spectral window.
Typical Wavelength-by-Wavelength Reference Monitoring Setup
Integrated Intensity
If you cannot perform the dual-beam type of reference monitoring, you can use the Integrated Intensity
method. However, this method is only effective if the drift from the detected light is uniform. Use this
option instead of the Single Point option if you can afford to use as your reference an area of the
wavelength region versus a single wavelength point.
Use the Integrated Intensity option if the following is true:
• You do not have two spectrometer channels identically configured
• The drift of the light source is uniform
• Your sample has a nonabsorbing wavelength region
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